Receptor Tyrosine Kinases Methods and Protocols [Paperback]

Receptor Tyrosine Kinases: Methods and Protocols explores on the most widely studied of the protein classes, known for their central role in several cellular functions and in a variety of human pathologies. Beginning with a section on activation and signaling of Receptor Tyrosine Kinases (RTKs), the book continues with chapters on aberrant expression of RTKs, trafficking and negative regulation, as well as RTKs as pharmacological targets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Receptor Tyrosine Kinases: Methods and Protocols contains an up-to-date overview of the most relevant and widely used methods employed in this field, providing detailed protocols that molecular and cellular biologists could easily adopt in their research programs on RTKs.

Part I: RTKs Activation and Signalling

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1. Analysis of Receptor Tyrosine Kinase (RTK) Phosphorylation by Immunoblotting

??????????? Martina McDermott and Norma ODonovan

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2. Analysis of Changes in Phosphorylation of Receptor Tyrosine Kinases: Antibody Arrays

??????????? Sweta Rani and Lorraine ODriscoll

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3. Analysis of Epidermal Growth Factor Receptor Dimerization by BS³ Crosslinking

??????????? Harmony F. Turk and Robert S. Chapkin

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4. Single-Molecule Optical Methods Analyzing Receptor Tyrosine Kinase Activation in Living Cells

??????????? Inhee Chung and? Ira Mellman

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5. Evaluation of tlƒƒ