Recombinant Proteins from Plants Methods and Protocols [Paperback]

This volume provides up-to-date scientific achievements from the worlds top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.

Part I: Vectors and Transformation

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1. Chloroplast Based Expression of Recombinant Proteins by Gateway? Cloning Technology

Johanna Gottschamel and Andreas L?ssl??????????????????????????????????????????????????????????????????????????????

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2.? Multigene Engineering in Rice Using High-Capacity Agrobacterium Tumefaciens BIBAC Vectors

Ruifeng He

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3. Virus-Derived Vectors for the Expression of Multiple Proteins in Plants

Pooja Saxena, Eva C. Thuenemann, Frank Sainsbury, and George P. Lomonossoff

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4. Transient Protein Expression by Agroinfiltration In Lettuce

Qiang Chen, Matthew Dent, Jonathan Hurtado, Jake Stahnke, Alyssaló'