Recombinant and In Vitro RNA Synthesis Methods and Protocols [Hardcover]

The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the inception of a new RNA project to the final sample ready for analysis. These experimental protocols combine established work-horse approaches with some of the latest innovative methods for RNA synthesis and purification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Recombinant and In Vitro RNA Synthesis: Methods and Protocols aids scientists in continuing to study the fascinating world of RNA.

Part of Springers Methods in Molecular Biology series, this collection of detailed protocols guides the reader from the start of a new RNA project to the final sample, with step-by-step protocols and expert tips on deploying catalytic RNAs in the laboratory.

1. Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold

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Luc Ponchon and Fr?d?ric Dardel

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2. Selective RNase H Cleavage of Target RNAs? from a tRNA Scaffold

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Luc Ponchon, Genevi?ve Beauvais, Sylvie Nonin-Lecomte and Fr?d?ric Dardel

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3. Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR