Reporter Gene Assays Methods and Protocols [Hardcover]

This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs.? Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications.? Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interested in learning more about reporter genes.

Preface&

Foreword&

Table of Contents&

Contributing Authors&

1. Genome-Edited Cell Lines for High-Throughput Screening

Patricia Dranchak, John Moran, Ryan MacArthur, Camila Lopez-Anido, James Inglese, and John Svaren

2. High-Throughput Firefly Luciferase Reporter Assays

Ellen Siebring-van Olst and Victor W. van Beusechem

3. Using the 2A Protein Co-Expression System: Multi-Cistronic 2A Vectors Expressing Gene(s) of Interest and Reporter Proteins

Garry A. Luke and Martin D. Ryan

4. Developing Mammalian ClCx