Research Methods in Neurochemistry Volume 2 [Paperback]

Section I Ultrastructure and Fragmentation of Neural Tissue.- 1 Bulk Separation of Neuronal Cell Bodies and Glial Cells in the Absence of Added Digestive Enzymes.- I. Introduction.- II. Bulk Isolation Procedures Requiring No Added Digestive Enzyme(s).- A. The Procedure Developed in the Authors Laboratory.- B. The Procedure of Nagata et al. (1971).- C. The Procedure of Iqbal and Tellez-Nagel (1972).- D. The Procedure of Jones et al. (1971).- III. General Procedural Comments.- IV. Cell Yield and Biochemical Characterization.- V. Applications in Cellular Neurochemistry.- A. Centrifugal Fractionation of Isolated Nerve Cell Bodies.- B. Metabolic and Enzyme Studies.- C. Miscellaneous Applications.- Acknowledgments.- References.- 2 Isolation of Brain Mitochondria.- I. Introduction.- II. Isolation of Brain Mitochondrial Fractions by Differential Centrifugaron.- A. General Aspects.- B. Homogenization Techniques.- C. Isolation Media.- D. Method I: Isolation of Bovine Brain Mitochondria (Stahl et al., 1963).- E. Method II: Isolation of Rat Brain Mitochondria (Ozawa et al., 1966).- III. Fractionation of Crude Mitochondrial Fractions by Density Gradient Centrifugation.- A. General Aspects.- B. Method III: Isolation of Rat Brain Mitochondria (Tanaka and Abood, 1963).- C. Method IV: Isolation of Rat Brain Mitochondria in a Continuous Gradient by Isopycnic Centrifugation (van Kempen et al., 1965).- IV. Criteria of Purity of Mitochondrial Fractions.- A. General Aspects.- B. Electron Microscopy.- C. Enzymes as Markers for Mitochondrial Fraction from Brain.- References.- 3 Preparation of Brain Mitochondrial Membranes.- I. Introduction.- A. Definitions and Abbreviations.- B. Membrane Markers.- II. Methods for Fragmentation.- A. Hypotonic Procedures.- B. Hypotonic Procedures with Sonication.- C. Phospholipases.- D. Digitonin.- III. Enzymatic and Other Criteria.- Acknowledgments.- References.- 4 Isolation and Study of Synaptic Vesicles.- R. M. Marchbanks.- I. Introduction: Types of Vesilc.