Retinal Degeneration Methods and Protocols [Hardcover]

This volume provides key updates on several methods from the first edition as well as including new novel techniques to address the most recent technological developments and their applications in retinal research Chapters guide readers through gene identification approaches, detailed protocols to generate functional retinal pigment epithelium cells, mouse retina and other animal models, fundus imaging and angiography, and cell-based treatment approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Retinal Degeneration: Methods and Protocols, Second Edition aims to ensure successful scientific work in the further study of this vital field.

Part I: Molecular Genetics and Tools

 

1      Identification and Analysis of Genes Associated with Inherited Retinal Diseases

Mubeen Khan, Zeinab Fadaie, St?phanie S. Cornelis, Frans P.M. Cremers, and Susanne Roosing

2      eQTL Analysis in Retinal Research

Felix Gra?mann

 

Khalid Rashid, Katharina Dannhausen, and Thomas Langmann

 

4      CRISPR/Cas9 Gene Editing In Vitro and in Retinal Cells In Vivo

Daniela Benati, Valeria Marigo, and Alessandra Rl3°