Reverse Genetics of RNA Viruses Methods and Protocols [Paperback]

This volume is a compilation of sixteen chapters that detail reverse genetics protocols. Reverse Genetics of RNA Viruses: Methods and Protocols guides readers through comprehensive protocols on RNA viruses, that were the most challenging to obtain and/or that were developed most recently. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Reverse Genetics of RNA Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. Reverse Genetics for Mammalian Orthoreovirus

Johnasha D. Stuart, Matthew B. Phillips, and Karl W. Boehme

 

2. Development and Characterization of an Infectious cDNA Clone of Equine Arteritis Virus

Udeni B. R. Balasuriya and Jianqiang Zhang

 

3. Reverse Genetics for Porcine Reproductive and Respiratory Syndrome Virus

Mingyuan Han, Hanzhong Ke, Yijun Du, Qingzhan Zhang, and Dongwan Yoo

 

4. Reverse Genetics of Zika Virus

Chao Shan, Xuping Xie, and Pei-Yong Shi

 

5. Efficient Reverse Genetic Systems for Rapid Genetic Manipulation of Emergent and

Pre-Emergent Infectious Coronaviruses

Adam S. Cockrell, Anne Beall, Boyd Yount, and Ralph Baric

 

6. Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus

Erica BlÃF