Rhinoviruses Methods and Protocols [Paperback]

Rhinoviruses: Methods and Protocols highlights the numerous molecular, cellular and in vivo tools now available to conduct human rhinovirus (HRV) research in an effort to increase understanding of the clinical disease caused by HRVs as well as the functions of its individual proteins and its replication. Human rhinoviruses are the major cause of common colds as well as being more recently recognized as the major viral cause of asthma and chronic obstructive pulmonary disease (COPD) exacerbations. The huge advancements in technical applications of biophysics and improvements in high-end imaging techniques have also had implications for HRV research among other infectious diseases. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Rhinoviruses: Methods and Protocols seeks to serve both professionals and novices with the latest technical advances in HRV research to ultimately enable the development of therapeutic approaches to combat HRV, and most importantly, its pathogenic effects in asthma exacerbation.

1. Classification and Evolution of Human Rhinoviruses

??????????????? Ann C. Palmenberg and James E. Gern

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2. Nested-RT-PCR and Multiplex RT-PCR for Diagnosis of Rhinovirus Infection in Clinical Samples

??????????????? Xuelin Yu and Reena Ghildyal

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3. Molecular Identification and Quantification of Human Rhinoviruses in Respiratory Samples

??????????????? Wai-Ming Lee, Kris Grindle, Rose Vrtis, Tressa Pappas, Fue Vang, Iris Lee, James E. Gern

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