Ribozymes Methods and Protocols [Hardcover]

Over the past decade, there have been a variety of fundamental insights into ribozymes which have changed the?perspective on RNA catalysis. In, Ribozymes: Methods and Protocols, researchers in the field detail many of the protocols and techniques which are now commonly used to study individual ribozymes and the characterization of ribozymes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Ribozymes: Methods and Protocols seeks to aid scientists in further understanding the catalytically active RNAs .

Ribozymes details protocols and techniques commonly used to study individual ribozymes and their characterizations. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and troubleshooting tips.

Ribozymes

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Table of Content

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1????????? Introduction

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Eric Westhof

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2????????? Characterization of Hammerhead Ribozyme Reactions

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Anne Kalweit, Rita Przybilski, Carsten Seehafer, Marcos de la Pe?a and Christian Hammann

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3????????? Mechanistic Analysis of the Hepatitis Delta Virus (HDV) Ribozyme: Methods for RNA Preparation, Structure Mapping, Solvent Isotope Effects, and Co-transcriptional Cleavage

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