RNA Interference, Editing, and Modification Methods and Protocols [Hardcover]

Part I. RNA Interference and Gene SilencingRNA Interference: Historical Overview and SignificanceMary K. MontgomeryDelivery of Double-Stranded RNA into Caenorhabditis elegansDawn Hull and Lisa TimmonsInduction and Biochemical Purification of RNA-Induced Silencing Complex From Drosophila S2 CellsAmy A. Caudy and Gregory J. HannonAnalysis of Gene Function in Trypanosoma brucei Using RNA InterferenceAppolinaire Djikeng, Shuiyuan Shen, Christian Tschudi, and Elisabetta UlluShort Hairpin Activated Gene Silencing in Mammalian CellsPatrick J. Paddison, Amy A. Caudy, Ravi Sachidanandam, and Gregory J. HannonGeminivirus Vectors for Transient Gene Silencing in PlantsNooduan Muangsan and Dominique RobertsonPosttranscriptional Gene Silencing in PlantsSusan Varsha Wesley, Chris Helliwell, Ming-Bo Wang, and Peter WaterhouseIdentification of microRNAs and Other Tiny Noncoding RNAs by cDNA CloningVictor Ambros and Rosalind C. LeePart II. RNA Editing and ModificationA Historical Perspective on RNA Editing: How the Peculiar and Bizarre Became MainstreamDonna J. KoslowskyIdentification of Substrates for Adenosine Deaminases That Act on RNADaniel P. MorsePurification and Assay of Recombinant ADAR Proteins Expressed in the Yeast Pichia pastoris or in Escherichia coliGillian M. Ring, Mary A. O'Connell, and Liam P. KeeganIsolation of an mRNA-Binding Protein Involved in C-to-U EditingCarri A. Gerber, Anne Relich, and Donna M. DriscollIn Vitro Assays for Kinetoplastid U Insertion-Deletion Editing and Associated ActivitiesKenneth Stuart, Reza Salavati, Robert P. Igo, Jr., Nancy Lewis Ernst, Setareh S. Palazzo, and Bingbing WangIdentification and Characterization of Trypanosome RNA-Editing Complex ComponentsKenneth Stuart, Aswini K.Panigrahi, and Achim SchnauferChimeric Templates and Assays Used to Study Physarum Cotranscriptional Insertional Editing In VitroElaine M. ByrneMethods for Analysis of Mitochondrial tRNA Editing in Acanthamoeba castellaniiAmanda J. Lohan and Michael W. GrayIn VitrolÅ