RNA Mapping Methods and Protocols [Hardcover]

In RNA Mapping- Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study RNA. These include protocols for the consequence of the emerging interest in the characterization of cellular RNAs urged by their potential use as diagnostic biomarkers or therapeutic targets. In particular, the biological relevance of microRNAs in human physiology and disease development is highlighted in the 16 chapters focused on methods for their physical and functional mapping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, RNA Mapping- Methods and Protocols provides instruction and inspiration for scientists who are facing the challenges of the discovery and/or functional characterization of RNA molecules for a wide variety of applications ranging from novel biomedical diagnostics to therapeutics and biomaterials.

Part I-Structural RNA Mapping

1.????? Full-Length Characterization Of Transcribed Genomic Regions

Marc Ren? Reboll, M. Lucrecia Alvarez, and Mahtab Nourbakhsh

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2.????? Rapid Mapping Of RNA 3?and 5?Ends

Victoria Zismann and Mahtab Nourbakhsh

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3.????? Single Nucleotide Mapping Of RNA 5?and 3?Ends

Mahtab Nourbakhsh

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4.????? RNA Secondary Structure Analysis

Mahtab ?Nourbakhsh

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5.????? Tertiary Structure Mapping Of Pri-Mirna17~92

Steven G. Chaulk and Richard P. Fahlman

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