RNA Methods and Protocols [Hardcover]

Recent insight into the transcripts generated from the mammalian genome (i.e. the transcriptome) has revealed that transcription is a far more complex phenomenon than previously thought. In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe the structure of messenger RNAs and non-coding RNAs that are transcribed by RNA polymerase II as the immediate gene products in mammalian cells. Focused on the structure of the RNA products of gene X and the mapping of proteins associated with these RNAs, the volume presents appropriate information for non-specialists in RNA biology. Written in the highly successful Methods in Molecular Biology series format, many chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, RNA: Methods and Protocols views the transcriptional landscape with an appreciation for the role that proteins play in the processing and interpretation of genetic information in an attempt to further our crucial knowledge of the many products and sophisticated regulatory networks that result from it.Part I: RNA Methods1. The Transcriptional LandscapeHenrik Nielsen2. Working with RNAHenrik Nielsen3. Synthesis of RNA by In vitro TranscriptionBertrand Beckert and Benoit Masquida4. Efficient Poly(A)+ RNA Selection Using LNA Oligo(T) CaptureNana Jacobsen, Jens Eriksen, and Peter Stein Nielsen5. Genome BrowsersElfar Torarinsson6. Web-Based Tools for Studying RNA Structure and FunctionAjish D. George and Scott A. Tenenbaum7. Northern Blotting AnalysisKnud Josefsen and Henrik Nielsen8. Rapid Amplification of cDNA Ends (RACE)Oladapo Yeku and Michael A. Frohman9. Fractionation of mRNA Based on the Length of the Poly(A) TailHedda A. Meijer and Cornelia H. de Moor10. Analysis of Mutations that Influence Pre-mRNA SplicingZhaiyi Zhanl“M