This detailed volume explores the continuing techniques ofstudying RNA-protein complexes and interactions as research in these areasexpand. After an introductory chapter,the book continues with ways to purify RNA-protein complexes assembled in cellsor in isolated cellular extracts, methods for measuring various biochemicalactivities of RNA-interacting proteins or ribonucleoproteins, biochemicalmethods for measuring direct RNA-protein contact, as well as various new orinnovative methods pertinent to the subject. Written for the highly successful Methods in Molecular Biology series,chapters contain brief introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date,
RNA-ProteinComplexes and Interactions: Methods and Protocols provides a set of usefulprotocols, both basic and advanced, designed to inspire researchers workingwith RNA and RNA-interacting proteins.
1. Characterization of RNA-ProteinInteractions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2
LindseySkrdlant and Ren-Jang Lin
2. Identification of mRNA-Interacting Factors byMS2-TRAP (MS2-Tagged RNA Affinity Purification)
Je-Hyun Yoon and Myriam Gorospe
3. Biotin-Streptavidin Affinity Purification ofRNA-Protein Complexes Assembled In Vitro
&nbslcl
![RNA-Protein Complexes and Interactions Methods and Protocols [Paperback]](/itemImage/2/9/0/5/2_1394512.jpg)