RNA-RNA Interactions Methods and Protocols [Paperback]

In this volume expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Thorough and intuitive, RNA-RNA Interactions: Methods and Protocols guides scientists investigating biological systems and studying RNA.

1. Identifying RNA Recombination Events and Non-covalent RNA-RNA Interactions with the Molecular Colony Technique

Helena V. Chetverina and Alexander B. Chetverin

?

2. RNA-directed Recombination of RNA in Vitro

Niles Lehman, Nilesh Vaidya, and Jessica A. M. Yeates

?

3. RNA-RNA Selex

B. Cho

?

4. Identification of Antisense RNA S0tem-loops that Inhibit RNA-protein Interactions using a Bacterial Reporter System

Kazuo Harada

?

5. Trans-activation of Large Ribozymes

Matthew B. Martin, Thomas L. Leeper, and Frank J. Schmidt

?

6. Native Purification and Labeling of RNA for Single Molecule Fluorescence Studies

Arlie J. Rinaldi, Krishna C. Suddala,and Nils G. Walter

?

7. Single Molecule Studies of RNA-RNA Interactions

Dongmei Yu, Peiwu Qin, and Peter V. Cornish

?

8. Modification IntelS