SARS- and Other Coronaviruses Laboratory Protocols [Paperback]

In 2003, the word coronavirus spread across the globe, somewhat further than the virus that sparked the panic. In this book, expert researchers examine these devastating viruses through 23 state-of-the-art, widely applicable protocols with minute detail. Comprehensive and cutting-edge, the book serves as an ideal guide for all virologists and especially for those working with coronaviruses. Written by international experts, this book is relevant to a wide array of professions.

In 2003, the word coronavirus spread across the globe, somewhat further than the virus that sparked the panic. In SARS- and Other Coronaviruses: Laboratory Protocols, expert researchers examine these devastating viruses through detailed laboratory protocols. Chapters deal with such subjects as detection and discovery of coronaviruses by nucleic acid and antibody-based approaches, virus isolation, propagation and titration, virus purification, structure analysis by electron cryomicroscopy, expression and crystallization of viral proteins, raising antibodies against viral proteins, manipulation of the coronavirus genome, and descriptions of how to investigate aspects of the cell surface for coronavirus receptors.

Comprehensive and cutting-edge, SARS- and Other Coronaviruses Laboratory Protocols serves as an ideal guide for all virologists and especially for those working with coronaviruses.

Preface Section 1. Detection and discovery of coronaviruses 1) A pancoronavirus RT-PCR assay for detection of all known coronaviruses, Leen Vijgen*, Elien Mo?s, Els Keyaerts, Sandra Li, and Marc Van Ranst 2) Detection of Group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactions, Leo L. M. Poon* 3) Detection and sequence characterisation of the 3 end of coronavirus genomes harbouring the highly conserved rna motif s2m, Christine Monceyron Jonassen* 4) RT-PCR detection of avian coronaviruses of gallil#º