Selenoproteins Methods and Protocols [Hardcover]

This volume aims at providing an update on state of the art methodologies to study various aspects of selenoprotein biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Selenoproteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Part I: Bioinformatic Tools for Selenoprotein Identification and Evolution

1. SECISearch3 and Seblastian: In Silico Tools to Predict SECIS Elements and Selenoproteins

Marco Mariotti

 

2. Selenoprofiles: A Computational Pipeline for Annotation of Selenoproteins

Didac Santesmasses, Marco Mariotti, and Roderic Guig?

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3. SelGenAmic: An Algorithm for Selenoprotein Gene Assembly

Liang Jiang and Qiong Liu

 

4. Selenocysteine tRNA^[Ser]Sec, the Central Component of Selenoprotein Biosynthesis: Isolation, Identification, Modification and Sequencing

Bradley A. Carlson, Byeong Jae Lee, Petra A. Tsuji, Paul R. Copeland, Ulrich Schweizer, Vadim N. Gladyshe, and Dolph L. Hatfield

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5. Identification and Characterization of Proteins that Bind to Selenoprotein 3' UTRs

Eric M. Cockman and Donna M. Driscoll

 

6. Specific Chemical Approaches for Studying Mammalil“)