Septins [Hardcover]

Septins provides established septin and molecular and developmental biologists and researchers new to the field with proven, state-of-art techniques and relevant historical background and theory to aid efficient design and effective implementation of experimental methodologies. Topics include the purification of septin proteins from diverse systems, their visualization in live cells, and their analysis by a variety of cutting-edge microscopy approaches.

• Provides the latest information on septins
• Includes both established and new technologies
• Brings together specialists from the field who contribute their expertise

1.?The Non-Opisthokont Septins: How Many There Are, How Little We Know About Them, and How We Might Learn More
Masayuki Onishi and John R. Pringle
2.?Preparing Recombinant Yeast Septins and Their Analysis by Electron Microscopy
Aur?lie Bertin and Eva Nogales
3.?A FRET-Based Method for Monitoring Septin Polymerization and Binding of Septin-Associated Proteins
Elizabeth A. Booth and Jeremy Thorner
4.?In Vitro Reconstitution of Septin Assemblies on Supported Lipid Bilayers
Andrew A. Bridges and Amy S. Gladfelter
5.?Visualization of In Vivo Septin Ultrastructures by Platinum Replica Electron Microscopy
Katy Ong, Tatyana Svitkina and Erfei Bi
6.?Assays for Genetic Dissection of Septin Filament Assembly in Yeast, From De Novo Folding Through Polymerization
Michael A. McMurray
7.?Investigation of Septins Using Infection by Bacterial Pathogens
Sina Krokowski and Serge Mostowy
8.?In Vivo Analysis of Septin Heteropolymer Rods and Higher-Order Structures in Filamentous Fungi
Amy Smith and Michelle Momany
9.?Live Cell Imaging of Septin Dynamics in Ustilago maydis
Sebastian BaumanlS: