Featuring a diverse array of model organisms and scientific techniques, Sirtuins: Methods and Protocols collects detailed contributions from experts in the field addressing this vital family of genes. Opening with methods to generate sirtuin biology tools, the book continues by covering methods to identify sirtuin substrates, to measure sirtuin activity, and to study sirtuin biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Comprehensive and easy to use,
Sirtuins: Methods and Protocols presents detailed protocols for sirtuin research that can be followed directly or modified to investigate new areas of sirtuin biology.
Part I: Methods to Generate Sirtuin Biology Tools
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1. Introduction: Sirtuins in Aging and Diseases
??????????? Leonard Guarente
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2. Sirtuins in Yeast: Phenotypes and Tools
??????????? Scott Tsuchiyama, Elizabeth Kwan, Weiwei Dang, Antonio Bedalov, and Brian K. Kennedy
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3. Studying the C. elegans Sirtuins
??????????? Mohan Viswanathan and Heidi A. Tissenbaum
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4. Genetic and Biochemical Tools for Investigating Sirtuin Function in Drosophila melanogaster
??????????? Jason G. Wood, Rachel Whitaker, and Stephen L. Helfand
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5. Generating Mammalian Sirtuin Tools for Protein-Interaction Analysis
??????????? Kathleen A. Hershberger, Jonathan Motley, Matthew D. Hirschey, and Kristin A. Anderson
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