Site-Specific Protein Labeling Methods and Protocols [Hardcover]

This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question.

1. How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag

B. Albert Griffin, Stephen R. Adams, and Roger Y. Tsien

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2. Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery

Lukas Leder

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3. Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins

Nicole J. Yang and Marlon J. Hinner

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4. Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes

Ivan R. Corr?a Jr.

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5. 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues

LilSH