In Situ Hybridization Protocols [Paperback]

In Situ Hybridization Protocols, Fourth Edition contains 21 protocols that utilize the in situ hybridization technology to document or take advantage of the visualization of specific RNA molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, In Situ Hybridization Protocols, Fourth Edition seeks to aid scientists in the further discovery of new RNA species and uncovering of their cellular functions.

1. Fixation/Permeabilization Procedure for mRNA in situ Hybridization of Zebrafish Whole-mount Oocytes, Embryos, and Larvae

Ricardo Fuentes and Juan Fern?ndez

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2. MicroRNA In Situ Hybridization on Whole Mount Preimplantation Embryos

Karen Goossens, Luc Peelman, and Ann Van Soom

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3. Whole-mount in situ Hybridization (WISH) Optimized for Gene Expression Analysis in Mouse Embryos and Embryoid Bodies

Eleni Dakou, Nele Vanbekbergen, Sara Corradi, Caroline R. Kemp, Erik Willems, and Luc Leyns

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4. Whole Mount in Situ Hybridization using DIG-labeled Probes in Planarian

Agnieszka Rybak-Wolf and Jordi Solana

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5. In Situ Hybridization on Whole-Mount Zebrafish Embryos and Young Larvae

Bernard Thisse and Christine Thisse

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6. LNA-based in Situ Hybridization Detection of mRNAs in Embryos

Diana K. Darnell and Parker B. Antin