Small Non-Coding RNAs Methods and Protocols [Hardcover]

This volume contains state-of-the-art methods tackling all aspects of small non-coding RNAs biology. ?Small Non-Coding RNAs: Methods and Protocols guides readers through customized dedicated protocols and technologies that will be of valuable help to all those willing to contribute deciphering the numerous functions of small non-coding RNAs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls.

Instructive and practical, Small Non-Coding RNAs: Methods and Protocols reaches out to biochemists, cellular and molecular biologists already working in?the field of RNA biology ?and to those just starting to study small non-coding RNAs.

Part I Introduction to Small Non-Coding RNAs

1. Small Non-Coding RNAs: A Quick Look in the Rear-View Mirror
Guillaume Clerget, Yoann Abel, and Mathieu Rederstorff

2. Alcoholic Precipitation of Small Non-Coding RNAs
Guillaume Clerget, Val?rie Bourguignon-Igel, and Mathieu Rederstorff

3. Quantification and Quality Control of a Small Non-Coding RNA Preparation
Virginie Marchand and Christiane Branlant

4. Impact of RNA Isolation Protocols on RNA Detection by Northern Blotting
Katrin Damm, Simone Bach, Katrin M. H. M?ller, Gabriele Klug, Olga Y. Burenina, Elena A. KlÃ^