Somatic Stem Cells Methods and Protocols [Hardcover]

This second edition volume expands on the previous edition with updated discussions on new genetic, molecular, and cellular methods used to study somatic stem cells. The chapters in this book focus on the isolation, classification, purity, and plasticity of these stem cells in a variety of organic tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Somatic Stem Cells: Methods and Protocols, Second Edition is a valuable resource for both novice and experienced molecular biologists, developmental biologists, tissue engineers, and geneticists who are interested in stem cell research and its potentials in regenerative medicine.

Preface&
Table of Contents&
Contributing Authors&

Part I Introduction

1. An Update on the Therapeutic Potential of Stem Cell
Pranela Rameshwar, Caitlyn A. Moore, Niloy N. Shah, and Caroline P. Smith 

Part II Stem Cell Study in Model Organisms and Human

2. Single-Step Plasmid Based Reprogramming of Human Dermal Fibroblasts to Induced Neural Stem Cells
Luis Azmitia and Philipp Capetian

3. Isolation and Analysis of Mesenchymal Progenitors of the Adult Hematopoietic Niche
Mayra Garcia, Lihong Weng, Xingbin Hu, and Ching-Cheng Chen

4. Identification and Isolation of Mice and Human Hematopoietic Stem Cells
Bijender Kumar and Srideshikan Sargur Madabushi

5. Identification and Characterization of Hair Folllsš