Sphingosine-1-Phosphate Methods and Protocols [Hardcover]

This fully updated edition explores methods involving sphingosine-1-phosphate (S1P), a bioactive lysophospholipid which has become the focus of much research interest as it has widespread developmental and physio-pathological actions, controlling events within the nervous, reproductive, gastrointestinal, vascular, respiratory, and immune systems, in addition to having a prominent role in cancer, early mammalian embryogenesis, and stem cells.  Here, worldwide experts in the S1P field describe in-depth techniques in an array of cell types and with various physiological applications, showcasing the important effects of S1P in development and in physiopathology.  As a volume in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the relevant materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and timely, Sphingosine-1-Phosphate: Methods and Protocols, Second Edition is another key resource for scientists working in this fast-moving and dynamic field.

1. Measuring Sphingosine-1-Phosphate/Protein Interactions with the Kinetic Exclusion Assay

            Jonathan K. Fleming and Jonathan M. Wojciak

 

2. An Improved Isoform-Selective Assay for Sphingosine Kinase 1 Activity

            Melissa R. Pitman, Lorena T. Davies, and Stuart M. Pitson

 

3. Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Plã©