Stem Cell Heterogeneity Methods and Protocols [Hardcover]

Given the variety of studies and data that have suggested the existence of heterogeneous populations or subpopulations of stem cells, this detailed volume examines different aspects of stem cell heterogeneity. This goes against the long-held tenet that stem cells, defined by their capacity for self-renewal and lineage development, comprised a homogenous population, thus providing the reader with a new avenue of exploration into the complex world of stem cell study. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

1. The Heterogeneity of Stem Cells: A Brief Overview

            Gy?rgyi Mqzes and Ferenc Sipos

 

2. Establishment and Characterization of Na?ve Pluripotency in Human Embryonic Stem Cells

            Sharat Warrier, Mina Popovic, Margot Van der Jeught, and Bj?rn Heindryckx

 

3. An Effective and Reliable Xeno-Free Cryopreservation Protocol for Single Human Pluripotent Stem Cells

            Guoliang Meng, Anna Poon, Shiying LilS