Stem Cell Renewal and Cell-Cell Communication Methods and Protocols [Hardcover]

This volume examines cell-cell interactions and stem cell renewal, two topics that are now inexorably linked as science strives to understand the stem cell niche and its function. Gathering a number of representative protocols, this detailed collection promises to provide readers with approaches for studying these complimentary aspects of stem cells. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Practical and reliable, Stem Cell Renewal and Cell-Cell Communication: Methods and Protocols will aid researchers in using these methods to advance their own studies.

1. Laboratory Control and Basic Culture Protocols for Stem Cell Self-Renewal

??????????? Jeong Mook Lim, Yoon Mi Jo, and Ji Yeon Ahn

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2. Advanced Fixation for Transmission Electron Microscopy Unveils Special Extracellular Matrix Within the Renal Stem/Progenitor Cell Niche

??????????? Will W. Minuth and Lucia Denk

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3. Multiplex Immunoassays for Quantification of Cytokines, Growth Factors, and Other Proteins in Stem Cell Communication

??????????? Ivona Valekova, Helena Kupcova Skalnikova, Karla Jarkovska, Jan Motlik, and Hana Kovarova

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4. Porous Membrane Culture Method for Expansion of Human Pluripotent Stem Cells

??????????? Jin-Su Kim, Seung-Taeh Hwang, and Soo-Hong Lee

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5. Tuning Differentiation Signals for Efficient Propagation and In Vitro Validation of Rat Embryonic Stem Cell Cultures

??????????? Stephen Meek, Linda Sutherland, and Tom Burdon