Stress Responses Methods and Protocols [Hardcover]

This volume focuses on detecting different cellular stresses, measuring pathological consequences within the cell, and investigating the role of cellular stresses in select diseases. In addition,?this book reviews the crosstalk between different stress pathways, stress responses during ageing, and targeting stress for regenerative medicine.?Written in the highly successful?Methods of Molecular Biology?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.?

Authoritative and instructive,?Stress Responses: Methods and Protocols?seeks to aid scientists to further study multiple stress pathways and outcomes triggered by such stresses. In depth knowledge of cellular stress will eventually lead to the development of novel therapeutics to prevent or treat related diseases.

Part I Assays for Detecting and Characterizing a Variety of Cellular Processes Under Cell Stress??

1. Methods for Studying ER Stress and UPR Markers in Human Cells
Donna Kennedy, Afshin Samali, and Richard J?ger

2. Assays for Induction of the Unfolded Protein Response and Selective Activation of the Three Major Pathways
Ananya Gupta, Danielle E. Read, and Sanjeev Gupta

3. Assays to Characterize Molecular Chaperone Functions in vitro
Martin Haslbeck and Johannes Buchner

4. Analysis of Heat Shock Factor Complex in Mammalian HSP70 Promoter
Mitsuaki Fujimoto, Ryosuke Takii, Naoki Hayashida, and Akira Nakai

5.?Immunofluorescence-Based Methods to Monitor DNA End Resection