Structures of Large RNA Molecules and Their Complexes [Hardcover]

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in RNA folding and dynamics, RNA-protein interactions and large RNPs.

• Continues the legacy of this premier serial with quality chapters on structures of large RNA molecules and their complexes

Part I: RNA Structure and Dynamics
1.?Native Purification and Analysis of Long RNAs
Isabel Chill?n, Marco Marcia, Michal Legiewicz, Fei Liu, Srinivas Somarowthu and Anna Marie Pyle
2.?Characterizing RNA Excited States Using NMR Relaxation Dispersion
Yi Xue, Dawn Kellogg, Isaac J Kimsey, Bharathwaj Sathyamoorthy, Zachary W Stein, Mitchell McBrairty, and Hashim M. Al-Hashimi
3.?Quantifying Nucleic Acid Ensembles with X-ray Scattering Interferometry
Xuesong Shi, Steve Bonilla, Daniel Herschlag and Pehr Harbury
4.?2-Aminopurine Fluorescence as a Probe of Local RNA Structure and Dynamics and Global Folding. Hairpins, Hinges, and Mg2+ ions in a 60 Nucleotide RNA
Michael J Rau and Kathleen B Hall
5.?Mod-seq: A High-Throughput Method for Probing RNA Secondary Structure
Yizhu Lin, Gemma E. May, and C. Joel McManus
6.?Reproducible Analysis of Sequencing Based RNA Structure Probing Data with User-Friendly Tools.
Lukasz Jan Kielpinski, Nikolaos Sidiropoulos and Jeppe Vinther
7.?Computational Methods for RNA Structure Validation and Improvement
Swati Jain, David C. Richardson, and Jane S. Richardson
8.?Structures of large RNAs and RNA Protein Complexes Towards Structure Determination of Riboswitches
Jason C. Grigg and Ailong Ke
Part II: RNA-Protein Interactions
9.?RRM-RNA Interactions
Tariq Afroz, Zuzana Cienikova, Antoine Cl?ry and lƒ"