Subcellular Proteomics From Cell Deconstruction to System Reconstruction [Hardcover]

This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. It examines the different levels of subcellular organization and their specific methodologies. In addition, the book includes coverage of systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system.

Introduction Section 1: Membrane proteomicsKeynotes on membrane proteomicsThierry Rabilloud Two-dimensional BAC/SDS-PAGE for membrane proteomicsRen? P. Zahedi, Jan Moebius and Albert Sickmann Microparticles: a new tool for plasma membrane sub-cellular proteomicLaurent Miguet, Sarah Sanglier, Christine Schaeffer, Noelle Potier, Laurent Mauvieux and Alain Van Dorsselaer. Lipid raft proteomics: more than just detergent-resistant membranesLeonard J. Foster and Queenie W. T. Chan Section 2: Organelle subproteomesOrganelle Proteome Variation Among Different Cell Types: Lessons from Nuclear Membrane ProteinsDeirdre M. Kavanagh, William E. Powell, Poonam Malik, Vassiliki Lazou and Eric C. Schirmer Synaptosome ProteomicsFengju Bai and Frank A. Witzmann Proteomic analysis of secreted exosomesChristine Olver and Michel Vidal Section 3: Characterization of supramolecular protein complexesFrom Protein-Protein Complexes to InteractomicsVincent Collura and Guillaume Boissy Supramolecular signalling complexes in the nervous systemMark O. Collins and Seth G.N. Grant Protein networks and complexes in photoreceptor ciliaRonald Roepman and Uwe Wolfrum Section 4: Subcellular systems biologySystems Biology and the Reconstruction of the Cell: from molecular components to integral functionFrank J. Bruggeman, Sergio Rossell, Karen van Eunen, Jildau Bouwman, Hans V. Westerhoff and Barbara Bakker Automated, Systematic Determination of Protein Subcellular Location using Fluorescence MicroscopyElvira Garc?a Osuna and Robert F. Murphy Systems biology of the Endoplasmic Reticululó$