This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward.
Part I: Mouse
1. Identification of Mouse T Follicular Helper Cells by Flow Cytometry
Alexandre Meli & Irah King
?2. Identification of Follicular T Helper Cells in Tissue Sections
Yanna Ding, John D. Mountz, and Hui-Chen Hsu
?3. Tracking Early T Follicular Helper Cell Differentiation In vivo
Dirk Baumjohann and K. Mark Ansel
?4. Tracking by Flow Cytometry Antigen-Specific Follicular Helper T-Cells In Wild-Type Animals After Protein Vaccination
Svetoslav Chakarov and Nicolas Fazilleau
?5. Retroviral Vector Expression in TCR Transgenic CD4+ T Cells
Youn Soo Choi and Shane Crotty
?6. Two-photon Microscopy For Imaging Germinal Centres and T Follicular Helper Cells
Menna R. Clatworthy
7. In vivo Induction of T-follicular Helper Cells by Modulation of Regulatory T Cell Function
James B. Wing and Shimon Sakaguchi
?8. Asslc*
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