Targeted Drug Delivery Methods and Protocols [Hardcover]

This volume looks at the latest techniques used to enable therapeutic molecules to be targeted for site-specific delivery. The chapters in this book discuss topics such as approaches to express biologically derived and synthetic nanocarriers; strategies to assist in nanocarrier targeting to specific cells and tissues; and methods for evaluating delivery and efficiency for new classes of agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Targeted Drug Delivery: Methods and Protocols is a valuable resource for anyone interested in understanding the diversity of methods used in this evolving field.

Table of Contents&

Contributing Authors&

Part I Drug Carrier Design

1. Synthesis of Cationic Polymer Libraries for Gene Delivery using Diglycidyl Ethers

Jacob Elmer, Thrimoorthy Potta, and Kaushal Rege

2. Generation of Ultra-Small PLGA Nanoparticles by Sequential Centrifugation

Xingwang Wu, Jiangbing Zhou, and Toral R. Patel

3. Construction of Bacteria-Based Cargo Carriers for Targeted Cancer Therapy

Mahama A. Traore, Ali Sahari, and Bahareh Behkam

4. Production of Extracellular Vesicles Loaded with Therapeutic Cargo

Tek N. Lamichhane and Stevl|