T-Helper Cells Methods and Protocols [Hardcover]

T-Helper Cells: Methods and Protocols presents a broad selection of cutting edge protocols that will enable the reader to capture the unique features of TH cells with tools developed for the isolation of TH cells from various tissues and subsequent analysis of their functional properties in vitro, ex vivo and in vivo. Chapters cover methods of isolating T cells from various tissues in mice, protocols for the analysis of T cell function and phenotype using various cutting edge technologies, methods allowing for the manipulation of T cell function in vitro and in vivo and in vivo models of diseases in which T cells play a central role in the pathogenesis. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, T-Helper Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further the study of this amazingly versatile and potent cell type.

Part I. Isolation of T Cells from the Tissues

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1. Isolation of T Cells from the Skin

Beate Lorenz and Esther von Stebut

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2. Isolation of Leukocytes from Mouse Central Nervous System

Iva Lelios and Melanie Greter

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3. Isolation of T Cells from the Gut

Sonja Rei?ig, Christopher Hackenbruch, and Nadine H?velmeyer

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4. T Cell Isolation from Mouse Kidneys

Isis Ludwig-Portugall and Christian Kurts

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