?In Tiling Arrays: Methods and Protocols,? expert researchers in the field detail many of the methods which are now commonly used to study tiling microarrays in genomic discovery . These include methods and applications for transcriptional regulation, expression, genetic and epigenetic regulation, as well as techniques and skills on tiling microarray data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
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Authoritative and practical, Tiling Arrays: Methods and Protocols ?seeks to provide scientists with a comprehensive and down to earth approach to maximize results.
This book details many of the methods that are now commonly used to study tiling microarrays in genomic discovery. It provides step-by-step detail essential for reproducible results and contains expert implementation advice.
Part I: OverView
1. A Brief Introduction to Tiling Microarrays: Principles, Concepts, and Applications
Christophe Lemetre and Zhengdong D. Zhang
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Part II: Transcriptional Regulation and Expression
2. Design of Tiling Arrays and their Application to Bacterial Transcriptome Analysis
Takeshi Akama, Kazuaki Nakamura, Akito Tanoue, and Koichi Suzuki
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3. Transcript Profiling in Arabidopsis with Genome Tiling Microarrays
Diana Coman, Wilhelm Gruissem, and Lars Hennig
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4. Genome-wide Analysis of Transcription Factor Binding Sites in Skeletal Muscle Cells using ChIP-seq
Chung-Il An al£Ù
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