Transport Organs Parts A and B [Paperback]

With contributions by numerous expertsVolume IV B.- 10  Perfusion of Isolated Mammalian Renal Tubules.- A. Introduction.- B. Experimental Techniques: Practical and Theoretical Considerations.- I. Isolation of Renal Tubule Segments.- II. Tubular Perfusion.- III. Perfusing and Bathing Solutions.- IV. Measurement of Net Volume Absorption.- 1. Collection Method.- 2. Crimped-End Method.- V. Assessment of Hydraulic Conductivity.- 1. The Unstirred Layer Problem.- 2. The Hydraulic Conductivity Coefficient.- VI. Electrical Measurements.- 1. Liquid Junction Potentials and Donnan Voltages.- 2. Ionic Dilution Potentials.- 3. Measurement of Transepithelial Resistance.- VII. Tracer Flux Measurements.- 1. Unidirectional Lumen-to-Bath Fluxes.- 2. Unidirectional Bath-to-Lumen Fluxes.- 3. Evaluating PDi From Tracer Fluxes.- 4. Transmembrane Fluxes.- C. Transport Properties of Isolated Nephron Segments.- I. Suitability of the In-Vitro Preparation.- II. The Proximal Tubule.- 1. Dissipative Transport Properties.- 2. Active Transport Processes.- 3. Mechanismus for Salt and Water Transport in the Superficial Pars Recta.- 4. Effective Luminal Hypotonicity and Isotonic Fluid Absorption.- 5. Heterogeneity of Tubular Structure and Function.- III. The Loop of Henle.- IV. The Distal Convoluted Tubule and Collecting Duct System.- 1. Distal Convoluted Tubule.- 2. Cortical Collecting Tubule.- 3. Water and Nonelectrolyte Permeation in the Cortical Collecting Duct: the Mechanism of ADH Action.- References.- 11  Metabolic Correlates of Tubular Transport.- A. Introduction.- B. Transport-Related Parameters of Metabolic Activity in Intact Cells.- I. General Aspects.- II. Heat Production in the Kidney.- III. O2 Consumption and CO2 Production in the Kidney.- IV. Effect of Metabolic Inhibitors on the Transepithelial Transport of Sodium.- V. Renal Substrate Metabolism in Relation to Renal Function.- 1. Uptake and Oxidation of Substrates in Relation to Sodium Transport.- 2. Substrate Dependence of Sodium Tl3#