Two-Hybrid Systems Methods and Protocols [Paperback]

Part I. General MethodsThe Two-Hybrid System: A Personal ViewStanley Fields and Paul L. BartelGrowth and Maintenance of YeastLawrence W. BergmanMedia Formulations for Various Two-Hybrid SystemsMichael Saghbini, Denise Hoekstra, and Jim GautschYeast Two-Hybrid Vectors and StrainsPhilip JamesHigh-Efficiency Transformation of Plasmid DNA into YeastRobin A. Woods and R. Daniel GietzQualitative and Quantitative Assessment of InteractionsMonica M. MontanoStrategies for Rescuing Plasmid DNA from Yeast Two-Hybrid Colonies,Alyson Byrd and Ren? St-ArnaudPart II. False PositivesTwo-Hybrid System and False Positives: Approaches to Detection and EliminationIlya G. Serebriiskii and Erica A. GolemisConfirming Yeast Two-Hybrid Protein Interactions Using In Vitro Glutathione-S-Transferase PulldownsDennis M. Kraichely and Paul N. MacDonaldTwo-Hybrid Interactions Confirmed by Coimmunoprecipitation of Epitope-Tagged ClonesLouie NaumovskiPart III. ApplicationsSmad Interactors in Bone Morphogenetic Protein SignalingXiangli Yang and Xu CaoProtein Interactions Important in Eukaryotic Translation InitiationKatsura Asano and Alan G. HinnebuschSteroid Receptor and Ligand-Dependent Interaction with Coactivator ProteinsSergio A. O?ateInteraction of Cellular Apoptosis Regulating Proteins with Adenovirus Anti-Apoptosis Protein E1B-19KThirugnana Subramanian and G. ChinnaduraiPart IV. Alternative StrategiesMammalian Two-Hybrid Assays: Analyzing Protein-Protein Interactions in the Transforming Growth Factor-_ Signaling PathwayXin-Hua Feng and Rik DerynckOne-Hybrid Systems for Detecting Protein-DNA InteractionsMary Kate Alexander, Brenda D. Bourns, and Virginia A.ZakianThe Split-Hybrid System: Uncoding Multiprotein Networks and Defining Mutations That Affect Protein InteractionsPhyllis S. Goldman, Anthony J. DeMaggio, Richard H. Goodman, and Merl F. HoekstraThree-Hybrid Screens: Inducible Third-Party SystemsBj?rn Sandrock, Franck Tirode, and Jean-Marc EglyThree-Hybrid Screens for RNA-Binding Proteinslã$