Unconventional Protein Secretion Methods and Protocols [Hardcover]

This volume presents relevantbackground information to understanding the molecular basis governingunconventional protein secretion (UPS), and in particular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic. Detailed chapters include an overview of conventional andunconventional secretory pathways along with multidisciplinary approaches andmethods used for UPS analysis in different organisms. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

 Authoritative and cutting-edge, UnconventionalProtein Secretion: Methods and Protocols will be usefulfor all interested in the secretory pathway field as well as applications incell biology, cell development, biomedical research, and healthcare.

PartI: Background

1.ER to Golgi Dependent Protein Secretion: The Conventional Pathway

CorradoViotti

Fanny Ng and Bor Luen Tang

3. UnconventionalProtein Secretion in Plants

Destiny J. Davis,Byung-Ho Kang, Angelo  S. Heringer,Thomas  E. Wilkop, and Georgia Drakakaki

Part II: UPSContemplates Multidisciplinary Approaches for Plants

4. ChemicalSecretory Pathway Modulation in Plant Protoplasts

FrancescaDe Marchis, Andrea Pompa, and Michele Bellucci

5.From Cytosol to the Apoplast: The HygromycinPhosphotransferase (HYG^R) Model in Arabidopsis

Haiyan Zhang and Jinjin Li

6.FollolÓ)