Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips.
- Includes resources for reagents
- Explains basic laboratory preparation
- Provides straightforward experimental protocols
- Incorporates fundamental analytical techniques
- Contains a troubleshooting guide
Chapter 1. Things to Consider Before Preparing to Undertake Your First Polymerase Chain Reaction
Chapter 2. Designing and Ordering Your Polymerase Chain Reaction Primers
Chapter 3. Analyzing Your Template DNA and/or PCR Product
Chapter 4. Quantitative PCR: Things to Consider
Chapter 5. Carrying Out Q-PCR
Chapter 6. Using PCR for Cloning and Protein Expression
Chapter 7. Polymerase Chain Reaction for Knocking Out Genes
A user friendly book that features step-by-step basic protocols, helpful resource recommendations, and basic troubleshooting tips, this approachable and practical guide explains the process for setting up and analyzing one's first PCR reaction
Sarah Maddocks, PhD, is a lecturer in Microbiology at Cardiff Metropolitan University, UK. Her research focuses on understanding the interactions between pathogens and their host during the infection process. The overarching aim of these studies is to understand how pathogens utilise or disrupt host processes and to explore and exploit novel ways of impairing these host-pathogen interactions to facilitate clearance olĂ)
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