Urothelial Carcinoma Methods and Protocols [Hardcover]

This volume details standard techniques for the characterization of urothelial carcinoma as well as methods to investigate mechanisms of carcinogenesis.? Chapters guide readers on cellular and animal models for urothelial carcinoma and related diseases, molecular analyses from body fluids, and new approaches to therapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Urothelial Carcinoma: Methods and Protocols hopes that the techniques described in this volume will contribute to the current upturn in research on urothelial carcinoma and to the application of its results in clinical practice.

PART I: Molecular Characterization

1.????? Analysis of Chromosomal Alterations in Urothelial Carcinoma
Donatella Conconi and Angela Bentivegna

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2.????? Analysis of Point Mutations in Clinical Samples of Urothelial Carcinoma
Mustafa Alamyar and Ellen C. Zwarthoff

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3.????? A Versatile Assay for Detection of Aberrant DNA Methylation in Bladder Cancer
Stella Tommasi and Ahmad Besaratinia

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4.????? Immunohistochemical Analysis of UC Tissues for Proliferation and Differentiation Markers
Michael Rose and Nadine Therese Gaisa

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5.????? Molecular Subtype Profiling of Urothelial Carcinoma Using a Subtype-Specific Immunohistochemistry Panel
Gottfrid Sj?dahl

PART II: ?Urothelial Carcinogenesis

6.????? Defining the Pathways of Urogenital Schistosomiasis-associated lór