Vibrio Cholerae Methods and Protocols [Hardcover]

This volume looks at the importance of V. cholerae as an infection agent, and explores the latest techniques used to study it. The chapters in this book cover topics such as the biology of V. cholerae; laboratory maintenance and culturing; various animals models developed to explain pathogenesis and factors for environmental survival; and holistic approaches that include proteomics, metabolomics, and high-throughput screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Vibrio cholerae: Methods and Protocols is a valuable resource for both novice microbiologists who want to focus their studies on V. cholerae, and experienced researchers and teachers who want to improve their knowledge and skills in this growing field.

Preface&

Table of Contents&

Contributing Authors&

1. Laboratory Culturing Techniques and Maintenance of Vibrio cholera

Kyle D. Brumfield, Bailey M. Carignan, and Mike S. Son

2. Genotypic and Phenotypic Assays to Distinguish Vibrio cholera Biotype

Kyle D. Brumfield, Bailey M. Carignan, and Mike S. Son

3. Preparation of Vibrio cholera Samples for RNA-seq Analysis

Jyl S. Matson

4. Random Transposon Mutagenesis of Vibrio cholera

Jyl S. Matson

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