Molecular biology and genetics techniques now dominate viral research in attempts to cure diseases such as AIDS. Viral Genome Methods is a practical guide to the newest molecular techniques, providing step-by-step protocols to be used in the laboratory. Recognized authorities and pioneers in viral research pass on their expertise to you.Retroviruses/HIV/RNA Viruses Retrovirus-Mediated Gene Transfer, B.A. Bunnell and R.A. Morgan Methods for Studying Genetic Variation of the Human Immunodeficiency Virus (HIV), G. Schochetman, S. Subbarao, and M.L. Kalish Methods for HIV/SIV Gene Analysis, A. Adachi, M. Kawamura, K. Tokunaga, and H. Sakai Analysis of Human Immunodeficiency Virus Reverse Transcriptase Function, V.R. Prasad, W.C. Drosopoulos, and Y. Kew Tat Activation Studies: Use of a Reporter Cell Line, E. Aguilar-Cordova, J. Chinen, and J.W. Belmont Characterization of HIV Nef Cell-Specific Effects, J.W. Marsh and S.K. De Detection, Quantitation, and Characterization of Human Retroviruses, S. Rasheed Quantitative Methods to Monitor Viral Load in Simian Immunodeficiency Virus Infections, S.I. Staprans, B.C. Corliss, J.L. Guthrie, and M.B. Feinberg Using Molecular Genetics to Probe the Immune Response to Vesicular Stomatitis Virus, C.S. Reiss, S.A. Oien, and S. Bartido DNA Viruses Transient Genetic Approaches to Studying Multifactor Processes in Complex DNA Viruses, G.S. Pari, A.C. Iskenderian, and D.G. Anders Epstein-Barr Virus: Gene Expression and Regulation, N.D. Sista and J.S. Pagano Functional Analysis of the Herpes Simplex Virus DNA Polymerase, P. Digard, W. Bebrin, C. Chow, and D.M. Coen Molecular Genetics of Adeno-Associated Virus, B.J. Carter Reconstitution of Adenovirus DNA Replication with Purified Replication Proteins, J. Dekker, F.E.J. Coenjaerts, and P.C. van der Vliet Index