This volume details protocols on virus-derived nanoparticles (VNPs) for a number of different applications. Chapters guide readers through the production of VNPs derived from plant, animal and bacterial viruses, prokaryotic and eukaryotic expression systems, encapsulation of heterologous materials within VNPs, and the modification of the outer surface of VNPs and how such modified VNPs can be developed into functional entities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Virus-Derived Nanoparticles for Advanced Technologies: Methods and Protocols aims to ensure successful results in the further study of this vital field.
PART I: PREPARING VIRUS AND VIRUS-DERIVED NANOARCHITECTURES AS CONTAINERS, CARRIER SCAFFOLDS, AND STRUCTURE-DIRECTING AGENTS: FROM PARTICLES TO FUNCTIONAL SUPER-ASSEMBLIES
1. The Production in Plants of Mosaic Turnip Crinkle Virus-like Particles Derived by Co-infiltration of Wild-Type and Modified Forms of Virus Coat Protein
Roger Castells-Graells, George P. Lomonossoff and Keith Saunders
2. Isolation and Characterization of Two Distinct Types of Unmodified Spherical Plant Sobemovirus-like Particles for Diagnostic and Technical Uses
Ina Balke, Gunta Resevi1a, and Andris Zeltins
3. RNA-directed Assembly of Tobacco Mosaic Virus (TMV)-Like Carriers with Tunable Fractions of Differently Addressable Coat Proteins
Sabine Eiben
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