In Vitro Methods in Toxicology [Paperback]

The volume brings together the once disparate knowledge of cultural techniques and thus provides an important reference work for industrial and academic toxicologists.This book describes tissue (cell and organ) culture techniques which could be used at a critical predevelopment stage to screen for drug-inducing toxicity or to analyse the molecular mechanisms of toxicity at a later stage in parallel with investigative studies.This book describes tissue (cell and organ) culture techniques which could be used at a critical predevelopment stage to screen for drug-inducing toxicity or to analyse the molecular mechanisms of toxicity at a later stage in parallel with investigative studies.First published in 1987, this book describes tissue (cell and organ) culture techniques which could be used at a critical predevelopment stage to screen for drug-inducing toxicity or to analyse the molecular mechanisms of toxicity at a later stage in parallel with investigative studies. This type of research has important ethical, financial and scientific implications. In particular, less animals and compound are required in in vitro research and more detailed information at the cellular and subcellular levels can be obtained. The volume brings together the once disparate knowledge of cultural techniques and thus provides an important reference work for industrial and academic toxicologists. It could also form a framework for discussion of potential improvements and modification of toxicity testing.Introduction G. B. Leslie and P. Johnson; Part I. Target Organ Toxicity: 1. In vitro methods in renal toxicology M. A. Snith, W. R. Hewitt and J. Hook; 2. Ototoxicity E. S. Harpur; 3. Cultured cell models for studying problems in cardiac toxicology N. Sperelakis and G. Bkaily; 4. Use of the isolated rat heart for the detection of cardiotoxic agents A. Cockburn; 5. Brain reaggregate cultures in neurotoxicological investigations C. K. Atterwill; 6. Application of thyroid cell culture to the studylC)