This detailed volume examines the latest techniques and protocols associated with zymography, the practice of detecting enzyme activity on electrophoretic gels. Sections cover subjects such as endopeptidase zymography, reverse zymography & in situ zymography, 2D zymography, as well as a variety of special cases. Written in the highly respected
Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Zymography: Methods and Protocols serves as an ideal aid for a broad array of researchers from the fields of biological and medicinal science, as well as scientists working in clinical and diagnostic medicine, medical genetics, agricultural entomology, genetic monitoring of environmental pollution, and forensic science.
Part I: Introduction
1. Zymography Principles
Jeff Wilkesman and Liliana Kurz
Part II: Endopeptidase Zymography
2. Serine Protease Zymography: Low-Cost, Rapid, and Highly Sensitive RAMA Casein Zymography
Hidetaro Yasumitsu
3. Cysteine Protease Zymography: Brief Review
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